Composite

Part:BBa_K5236016:Design

Designed by: Hening Guo, Yangzihan Chu   Group: iGEM24_BASIS-China   (2024-10-01)


pET28a-pelB-IsPETase(H104W)-6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


Design Notes

For expressing the sequence, signal peptides and lac operator are suggested to consider when designing a pathway.



Source

Mutation site predicted by AI

References